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Global Regenerative Trade

LIPO-STEM DUO

Adipose tissue MSCs microfragmentation and purification kit

LIPO-STEM DUO is a closed-circuit single-use kit for adipose tissue microfragmentation and purification without any centrifuge and with a minimal manipulation.

The filtering system consistently microfragments the adipose tissue while manteining its biological properties and maximizing the regenerative potential.

 

The entire processing phase of the liposuctioned tissue occurs inside the device thanks to continuous saline solution washing. This allows to reduce the cellular stress eliminating any traumatic action that may damage the extracellular matrix and its essential trophic and anti-inflammatory function.

The collection and processing bag is equipped with two filters:

  • the first filter microfragments the adipose tissue, while retaining the eventual fibrotic tissue
  • the second filter with denser mesh retains the reduced (microfragmented) adipose tissue that is washed with saline solution eliminating all the oily and blood residues which might cause inflammation of the treated tissues.

The final microfragmented and purified product is an autologous adipose tissue that keeps the biological properties of the original tissue intact and can easily be injected even through very thin needles.

LIPO-STEM DUO PRODUCT CHARACTERIZATION
PERFORMED BY PROF. CARLO VENTURA

Director of the Laboratory of Molecular Biology and Stem Cell Bioengineering of the National Institute of Biostructures and Biosystems

KEY ADVANTAGES


 
  • No centrifugation required

  • Adipose tissue microfragmentation and purification

  • Preservation of extracellular matrix and stromal niche components

  • Total purification of blood and oily waste as potential cause of inflammation

  • Minimal manipulation thanks to a simple and reproducible technique in a single surgical time

  • Sampling and processing in a sterile closed-circuit environment

MEDICAL PROFESSIONALS

LIPO-STEM DUO is a closed-circuit single-use kit for adipose tissue microfragmentation and purification without any centrifuge and with a minimal manipulation.

FIELDS OF APPLICATION

SPINE SURGERY

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MAXILLO- FACIAL SURGERY

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Aurafix Nose Supporter

AESTHETIC AND RECONSTRUCTIVE SURGERY

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PAIN MANAGEMENT

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WOUND CARE

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SURGICAL TECHNIQUE​

How does it works?

SURGICAL TECHNIQUE

1. Infiltrate the Klein solution in the elected harvest site using the 16 G Klein cannula

2. Harvest the adipose tissue from the anesthetized area using the 13 G lipoaspiration cannula.

3. Connect a 2L saline bag to the device’s “WASH IN” valve and fill the device.

4. Insert the harvested adipose tissue in the device’s “LIPO IN” valve.

5. The collection and processing bag is equipped with two filters: the first filter microfragments the adipose tissue, while retaining the eventual fibrotic tissue.

6. Support the purification of the adipose tissue using the processing spatula. This will gently microfragment and purify the adipose tissue.

 

7. The second filter with a denser mesh retains the microfragmented adipose tissue and discharges the pro-inflammatory contents and liquids in the waste bag.

8. The eventual fibrotic tissue is retained in the first filter, allowing to obtain a high qualitative adipose tissue.

9. Continue to gently move the processing spatula on the filter to facilitate the complete washing of the tissue.

 

Once the final product acquires a clear yellow color, stop the saline solution flow and gently remove the excess of water using the processing spatula.

 

10. With the help of the processing spatula retrieve the purified and microfragmented adipose tissue from the dedicated “LIPO OUT” valve and use as needed.

COMPONENTS

– LIPO-STEM DUO device

The device is supplied ready-to-use with a 400 ml capacity processing bag and a 2 lt waste collecting bag. The kit includes a processing spatula to facilitate the tissue purification.

The system has 3 valves:

       – WASH IN: to connect the saline solution

      – LIPO IN: to transfer the harvested adipose tissue into the device

      – LIPO OUT: to extract the microfragmented and purified adipose tissue.

 

– Filtering system

The sophisticated filtering system inside the processing and filtering bag is equipped with two filters:

  • the first filter microfragments the adipose tissue, while retaining the eventual fibrotic tissue
  • the second filter with denser mesh retains the reduced (microfragmented) adipose tissue that is washed with saline solution eliminating all the oily and blood residues which might cause inflammation of the treated tissues. 

– 16 G Klein solution cannula with 60 ml syringe

The kit includes:

  • two 60 ml syringes for Klein solution infiltration
  • one 16 G cannula with atraumatic tip and three side holes for Klein solution infiltration.

– 13 G Atraumatic lipo-aspiration cannula with 60 ml Vaclok syringe

The kit includes:

  • two 60 ml Vaclok syringes for lipoaspiration
  • one 13 G lipoaspiration cannula with atraumatic tip and three ultrasharp side holes that allow to harvest a high qualitative and vital adipose cluster.
Vivostat Logo

THE VIVOSTAT® SYSTEM

The uniqueness of the Vivostat® system is a novel patented biotechnological process that enables reliable and reproducible preparation of autologous Fibrin Sealant or Platelet Rich Fibrin (PRF®) without using cryoprecipitation and without the need for a separate thrombin component.

THE FULLY AUTOMATED VIVOSTAT® SYSTEM CONSISTS OF THREE COMPONENTS: