Global Regenerative Trade

Plasma Ultrafiltrator

First-class concentrator of proteins and platelets


Platelets and mononucleated cells have numerous advantages as biological products for the treatment of many pathologies.

They are easily prepared autologous products, cause minimal complications, and have a broad range of potential actions.

Platelets, mononucleated cells and the liquid portion (plasma) of PRP simultaneously release many proteins and factors with different anabolic functions that reduce inflammation and promote angiogenesis and tissue regeneration. Wound healing in response to injury involves the coordination of a large number of complex cellular and molecular events within the body.

This response is defined by the need for cells to travel to the site of injury, proliferate, form blood vessels, and, eventually, extracellular matrix (ECM) to restore both the structure and the function of the damaged tissue.

The aqueous part of PRP is plasma, which is an acellular component containing proteins that are important for tissue regeneration.

These proteins are involved in the tissue healing process and enable anti-inflammatory effects, clot stabilization, anti-microbial actions, cell-matrix adhesion, and tissue synthesis while acting overall as a ubiquitous broad-spectrum proteinase inhibitor.

These cellular processes are driven by a wide variety of proteins, growth factors, and cytokines that act to control cell functions.

One of the most important plasma cytokines is a2M.

The alpha-2-macroglobulin molecule is synthesized mainly in the liver, but also locally by macrophages, fibroblasts, and it is the largest major nonimmunoglobulin protein in plasma. Alpha 2 macroglobulin acts as an antiprotease and can inactivate an enormous variety of proteinases.

It works as an inhibitor of coagulation by inhibiting thrombin. Alpha-2-macroglobulin may act as a carrier protein because it also binds to numerous growth factors and cytokines, such as platelet-derived growth factor, basic fibroblast growth factor, TGF-β, insulin, and IL-1β.

The α2M offers great potential for the regulation of cytokine homeostasis in blood and tissue, a critical point in the pathogenesis of several diseases.

Mechanical concentration accessory,
compatible with 20 ml syringes.

α2M is known as an ubiquitous broad-spectrum proteinase inhibitor.

α2M traps the proteinases released by granu- locytes during inflammation, such as matrix metalloproteinases (MMPs).

α2M is a master inhibitor of many types of cartilage- degrading enzymes, is an exciting and potential candidate for OA treatment.

α2M may play an important role in regulating inflammatory reactions in joint diseases, not only because of its action as an inhibitor of proteases, but also because of its potential to modulate several cell types involved in joint inflammation.

α2M is also involved in the immune defense reactions

with a role of protection against toxic effects by an over production of pro-inflammatory cytokines during inflammation.

But the plasma protease inhibitor a2M is not present in sufficient concentrations to inactivate the high levels of catabolic factors found in OA synovial fluid. Findings suggest that supplemental intra-articular A2M

provides chondral protection in post-traumatic OA.



What is it for?

ProtSmart™ is a class IIa medical device, designed for plasma concentration and blood components. ProtSmart™ is a hollow capillary membrane device that can be used downstream of the blood product preparation method, in order to concentrate more platelets and plasma PRP proteins or even simply the plasma proteins of PPP. The result is a concentration of solutes over a certain molecular weight (greater than the size of the pores) on one side of the membrane, and a loss of liquids and small solutes (smaller than the size of the pores) on the other.

This step is particularly useful if you want to obtain a blood product of high concentrations in small volumes.

In fact, the three ProtSmart™ models available make it possible to obtain autologous preparations of just a few ml, with platelet and protein concentrations that even reach 4-6 times the basal concentrations in whole blood.

ProtSmart™ can concentrate PRP and PPP with a volume reduction of the order of 70-75%.

During this push phase, the pressure gradient created between the two sides of the hollow capillary membrane allows plasma water to escape from its pores.

At the end of the procedure, a simple aspiration enables recovery of the concentrated plasma, from inside the ProtSmart™ device.

This kit is designed to provide at least 6x concentration of A2M, cells and Fibrinogen Proteins.

ProtSmart™ was born from the combination of capillary membrane filtration technologies and the concentration of blood products.

Thanks to a specially developed capillary membrane morphology with an average pore size of 15,000 Da, it is possible to eliminate plasma water thus concentrating the platelets, mononucleated cell, bone marrow and growth factors like alpha 2 macroglobulins present in the plasma.

A2M also binds up pro-inflammatory molecules TNF-a, TGF-b, and IL-1b7.

While IRAP (Interleukin-1 receptor antagonist protein) is present at much higher levels in bone marrow aspirate.

Technical Features

Instructions for use

After checking the product and packaging integrity, proceed as explained below.

The concentration procedure takes place through the use of at least 1 syringe (recommended volume 20 ml) and the mechanical accessory (Fig. A) and the entire procedure is shown in the figure sequence 1-7. Fig A: Front view of the mechanical accessory with the 20 ml syringe already connected (the syringe has to be pre-filled with the fluid to be concentrated: max. loading volume for ProtSmart™ 2 is 8 ml, max loading volume for ProtSmart™ 6 is 16 ml).

Once the syringe is filled with the fluid to be concentrated, it should be connected in sterile mode with the ProtSmart™ device. The system can be then placed in the relevant slot on to the mechanical accessory for concentration.

Turn the knob until it touches the syringe plunger. Fig. B: Rear view of the mechanical accessory for concentration with the syringe of 20 ml (pre-filled) already mounted and connected. It is recommended to check the position of the 3-way stopcock at the bottom; this should be positioned to enable a one-way flow between the syringe and ProtsmartTM device. Once the whole system is assembled, fit the protective cover provided together with the mechanical accessory on the base of the same (the cover is not shown in the pictures below to facilitate understanding of the procedure).



Bone and soft tissue regeneration


Anti-aging, bio-revitalization and biological filler


Musculo-skeletal iniuries, osteoarthrosis, tendonopathy cartilage regeneration


Acute and chronic ulcers


Scars, alopecia and hair transplantation


Scleroderma, Peyronie’s disease, erectile dysfuncion, tissue rejuvenation


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Wang et al. Identification of alpha-2- macroblobulin as a master inhibitor of cartilage-degrading factors that attenuates the progression of post traumatic osteoarthritis. Arthritis & Rheumatology 2014; 66(7): 1843-1853

Cassano et al. Bone marrow concentrate and platelet-rich plasma differ in cell distribution and interleukin1receptor antagonist protein concentration. Knee Surg Sports Tramotol Arthrosc, 1 Feb 2016

AhmedA, et al. Alpha-2-Macroglobulin: A Physiological Guardian, Journal of cellular physiology, 2013 – Wiley

Barrientos et al., Growth factors and cytokines in wound healing. Wound Repair Regen. 16, 585–601. 2008

Karli et al. “Autologous Regenerative Therapies: Rapid Concentration of Progenitor Cells, Platelets, and Proteins at the Point-of-Care,” TERMIS annual meeting, September 2015, Boston, MA

Hegde et al. “A Prospective Comparison of 3 Approved Systems for Autologous Bone Marrow Concentration Demonstrated Nonequivalence in Progen itor Cell Number and Concentration,” J Ortho Trauma, Vol. 28, October 2014

Julia Etalain et al. An Optimized Protocol for Platelet Rich Plasma Preparation to Improve its Androgenic and Regenerative Properties. Nature: Scientific Reports (2018) 8:1513

Xie et al. Biology of platelet-rich plasma and its clinical application in cartilage repair, Arthritis Research and Therapy 2014, 16:204

Sundman EA, Cole BJ, Fortier LA. Growth factor and catabolic cytokine concentrations are influenced by the cellular composition of platelet-rich plasma. Am J Sports Med 2011; 39: 2135-2140

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Lee HW, Choi KH, Kim JY, et al. Proteomic classification and identification of proteins related to tissue healing of platelet-rich plasma. Clin Orthop Surg 2020; 12: 120-129

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Protein-enriched Platelet-Rich Plasma (PEFPRP) a NewProducts for Tissue Regeneration Developed Through the Ultrafiltration of PRP – Preclinical Study Laura Mazzucco, Valeria Balbo1, Simona Martinotti, Elia Ranzato, Mauro Patrone, Marcello Manfredi2, Roberto Guaschino Frontiers 2021

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Baltzer A et al (2013): A new treatment for hip osteoarthritis: clinical evidence for the efficacy of autologous conditioned serum; Orthopedic Reviews 2013, 5:e13: 59-64

Baselga GEJ, Hernández Trillos PM (2015): Treatment of Osteoarthritis of the Knee with a Combination of Autologous Conditioned Serum and Physiotherapy: A Two-Year Observational Study. PLOS One 2015 Dec, 28:10(12)

Godek P (2016): Use of Autologous Serum in Treatment of Lumbar Radiculopathy Pain. Pilot Study. Orthop. Traumatol. Rehabil. 2016 Jan -Feb; 18(1):11-20

Vivostat Logo


The uniqueness of the Vivostat® system is a novel patented biotechnological process that enables reliable and reproducible preparation of autologous Fibrin Sealant or Platelet Rich Fibrin (PRF®) without using cryoprecipitation and without the need for a separate thrombin component.